\u7531\u4e8e\u671f\u520a\u6240\u6536\u5f55\u7684\u6587\u7ae0\u5185\u5bb9\u7684\u4e0d\u540c\uff0c\u6211\u4eec\u5c06\u671f\u520a\u5212\u5206\u4e3a\u4e13\u4e1a\u671f\u520a\u548c\u7efc\u5408\u671f\u520a\u4e24\u5927\u7c7b\u3002\u4e13\u4e1a\u671f\u520a\u5373\u53ea\u6536\u5f55\u67d0\u4e00\u4e13\u4e1a\u6216\u67d0\u4e00\u9886\u57df\u7684\u4e00\u7c7b\u671f\u520a\uff1b\u800c\u7efc\u5408\u671f\u520a\u5219\u662f\u5bf9\u6536\u5f55\u7684\u6587\u7ae0\u4e13\u4e1a\u9886\u57df\u6ca1\u6709\u5b58\u5728\u9650\u5236\u7684\u4e00\u7c7b\u671f\u520a\u3002\u90a3\u4e48\uff0c\u5f53\u4f60\u60f3\u8981\u6295\u9012\u4e00\u7bc7SCI\u6587\u7ae0\u7684\u65f6\u5019\uff0c\u662f\u9009\u62e9\u4e13\u4e1a\u671f\u520a\u8fd8\u662f\u7efc\u5408\u671f\u520a\u5462\uff1f<\/p>\n
\u7efc\u5408\u6027\u7684\u6742\u5fd7\u901a\u5e38\u4f1a\u6bd4\u8f83\u6ce8\u91cd\u65b9\u6cd5\u5b66\uff0c\u6bd4\u5982meta\uff0c\u4ed6\u4eec\u5ba1\u7a3f\u7684\u5f88\u53ef\u80fd\u6709\u7edf\u8ba1\u5b66\u5bb6\u4e0e\u4e34\u5e8a\u6d41\u884c\u75c5\u5b66\u5bb6\u53c2\u4e0e\uff0c\u53ef\u80fd\u5bf9\u7eb3\u5165\uff0c\u65b9\u6cd5\u7b49\u65b9\u9762\u8981\u6c42\u751a\u9ad8\uff0c\u53ef\u80fd\u4f1a\u5bf9method\u628a\u5173\u5f88\u4e25;\u800c\u4e13\u4e1a\u6742\u5fd7\u53ef\u80fd\u5bf9\u7acb\u9898\uff0c\u4e5f\u5c31\u662fintroduction\uff0cresults, discussion\u6bd4\u8f83\u611f\u5174\u8da3;\u4f46\u5f80\u5f80\u5f88\u5927\u7a0b\u5ea6\u4e0a\uff0c\u65e0\u6cd5\u786e\u8ba4\u6b64\u7acb\u9898\u7684\u610f\u4e49\u6709\u591a\u5927\uff0c\u4e8b\u5b9e\u4e0a\u7acb\u9898\u610f\u4e49\u5927\u5c0f\uff0c\u4e5f\u662f\u89c1\u4ec1\u89c1\u667a\u3002\u6545\u800c\uff0c\u5728SCI\u7684\u5199\u4f5c\u4e2d\uff0c\u6211\u4eec\u5c31\u8981\u6839\u636e\u81ea\u5df1\u6240\u9009\u62e9\u7684\u671f\u520a\u7c7b\u578b\u800c\u5728\u5199\u4f5c\u7684\u65f6\u5019\u6709\u6240\u4fa7\u91cd\u3002<\/p>\n
\u7efc\u5408\u671f\u520a\u4fa7\u91cd\u65b9\u6cd5\uff0c\u90a3\u4e48\u65b9\u6cd5\u90e8\u5206\uff0c\u6211\u4eec\u5e94\u8be5\u600e\u4e48\u5199\uff1f<\/p>\n
\u201c\u65b9\u6cd5\u201d\u90e8\u5206\u5e94\u5305\u542b\u8db3\u591f\u7684\u4fe1\u606f\u4ee5\u4f9b\u5176\u4ed6\u7814\u7a76\u8005\u51c6\u786e\u91cd\u590d\u4f60\u63cf\u8ff0\u7684\u8bd5\u9a8c;\u5982\u679c\u5176\u4e2d\u6709\u5173\u952e\u4fe1\u606f\u9057\u6f0f\uff0c\u522b\u4eba\u5c31\u65e0\u6cd5\u5b8c\u5168\u91cd\u590d\u4f60\u7684\u5b9e\u9a8c\u6761\u4ef6;\u8fd9\u4f1a\u5f15\u8d77\u7ed3\u679c\u4e0d\u4e00\u81f4\uff0c\u6709\u53ef\u80fd\u9020\u6210\u8bef\u4f1a\uff0c\u751a\u81f3\u8fd8\u4f1a\u88ab\u4eba\u6307\u8d23\u9020\u5047\u3002\u6240\u4ee5SCI\u65b9\u6cd5\u90e8\u5206\u8981\u529b\u6c42\u5168\u9762\u3002<\/p>\n
\u201c\u65b9\u6cd5\u201d\u90e8\u5206\u5e94\u8be5\u4f7f\u7528\u8fc7\u53bb\u65f6\u6001\uff0c\u4f8b\u5982\uff1a\u201csections were stained with\u201e\u201dand \u201cdata were analyzed\u00a0using\u201e\u201d\u3002\u4f46\u662f\u5728\u63d0\u53ca\u56fe\u8868\u65f6\u5e94\u4f7f\u7528\u73b0\u5728\u65f6\uff0c\u4f8b\u5982\uff1a\u201cThe patient\u2019s\u00a0clinical characteristics are\u00a0listed in Table 1\u201d\u3002\u6b64\u5916\uff0c\u5f53\u4f60\u5728\u53d9\u8ff0\u67d0\u4e2a\u4e8b\u7269\u7684\u5b9a\u4e49\u6216\u5f53\u524d\u5bf9\u5b83\u516c\u8ba4\u7684\u770b\u6cd5\u65f6\u4e5f\u8981\u7528\u73b0\u5728\u65f6\uff0c\u4f8b\u5982\uff1a\u201cthe cells were\u00a0subjected to hypoxia, which induces HIF-1expression\u201e\u201d\u3002\u8fd9\u53e5\u8bdd\u4e2d\uff0c\u867d\u7136\u5b9e\u9a8c\u90e8\u5206\u662f\u7528\u7684\u8fc7\u53bb\u65f6\uff0c\u4f46\u7531\u4e8e\u7f3a\u6c27\u5bfc\u81f4HIF-1\u662f\u4e00\u4e2a\u666e\u904d\u73b0\u8c61\u800c\u4e0d\u662f\u9650\u5b9a\u4e8e\u672c\u6587\u7684\u7ed3\u679c\uff0c\u56e0\u6b64\u540e\u9762\u534a\u53e5\u5e94\u8be5\u7528\u73b0\u5728\u65f6\u3002<\/p>\n
\u8981\u5217\u51fa\u6240\u6709\u8bd5\u5242\u7684\u4f9b\u5e94\u5546\u4ee5\u53ca\u8bbe\u5907\u7684\u5382\u5bb6;\u6709\u7684\u671f\u520a\u8fd8\u8981\u6c42\u5217\u51fa\u5176\u5730\u5740\uff0c\u5373\u56fd\u5bb6\u3001\u5dde(\u5982\u4e3a\u7f8e\u56fd)\u548c\u5e02\u3002\u53d9\u8ff0\u8bbe\u5907\u3001\u8bd5\u5242\u76d2\u3001\u8bd5\u5242\u7684\u65f6\u5019\u5e94\u8be5\u7528\u5e38\u7528\u672f\u8bed\u6765\u5177\u4f53\u63cf\u8ff0\uff0c\u4e0d\u8981\u53ea\u7528\u5382\u5546\u7684\u4e13\u95e8\u672f\u8bed\u6216\u53ea\u5199\u578b\u53f7\u3002\u6bd4\u5982\uff0c\u4e0d\u8981\u5199\u6210\u201cAbsorbance in each well was measured at a wavelength of 492 nm usinga BeckmanCoulter AD 340C\u201d\uff0c\u56e0\u4e3a\u5927\u591a\u6570\u4eba\u90fd\u5bf9Beckman Coulter AD340C\u6ca1\u6709\u4efb\u4f55\u6982\u5ff5;\u5e94\u8be5\u5199\u6210\uff1a\u201cAbsorbance in each well was measured at a wavelengthof 492 nm using a multi-well plate reader (AD 340C, BeckmanCoulter)\u201d\uff0c\u6216\u8005\u201cAbsorbance in each well was measured at a wavelength of492 nm using an AD 340C multi-well plate reader (Beckman Coulter Inc,Fullerton, CA, USA)\u201d\u3002<\/p>\n
\u201c\u65b9\u6cd5\u201d\u90e8\u5206\u53ea\u80fd\u5305\u62ec\u6700\u7ec8\u5f97\u51fa\u7ed3\u679c\u7684\u5b9e\u9a8c\u65b9\u6cd5\u3002\u5982\u679c\u67d0\u4e2a\u8bd5\u9a8c\u5931\u8d25\u4e86\u6216\u8005\u6ca1\u80fd\u63d0\u4f9b\u4f60\u9700\u8981\u7684\u7ed3\u679c\uff0c\u800c\u4e14\u4f60\u5df2\u7ecf\u51b3\u5b9a\u8bba\u6587\u4e2d\u4e0d\u63d0\u8fd9\u4e9b\u7ed3\u679c\uff0c\u90a3\u4e48\u4e5f\u6ca1\u6709\u5fc5\u8981\u53bb\u8c08\u5176\u8bd5\u9a8c\u65b9\u6cd5\u3002\u7528\u9002\u5f53\u7684\u5b50\u6807\u9898\u628a\u5404\u79cd\u4e0d\u540c\u76ee\u7684\u7684\u6750\u6599\u548c\u65b9\u6cd5\u5206\u7c7b\u3002\u5982\u679c\u671f\u520a\u8bbe\u6709\u201c\u8865\u5145\u65b9\u6cd5\u201d(SupplementaryMethods)\u90e8\u5206\uff0c\u5219\u53ef\u7528\u8fd9\u90e8\u5206\u6765\u8be6\u8ff0\u7ec6\u8282\uff0c\u5e76\u8ba9\u7eb8\u9762\u520a\u51fa\u7684\u201c\u65b9\u6cd5\u201d\u90e8\u5206\u4fdd\u6301\u7b80\u77ed\u3002\u65b0\u65b9\u6cd5\u5e94\u8be5\u8be6\u7ec6\u53d9\u8ff0\u4ee5\u4fbf\u4ed6\u4eba\u91cd\u590d;\u6807\u51c6\u548c\u5e38\u7528\u6280\u672f\u53ea\u987b\u5f15\u7528\u6587\u732e\u5373\u53ef\uff0c\u4f46\u5982\u679c\u4f60\u7684\u65b9\u6cd5\u548c\u6587\u732e\u6709\u5dee\u5f02\u4e4b\u5904\u5e94\u4e88\u8bf4\u660e\u3002\u5c24\u5176\u8981\u4fdd\u8bc1\u6240\u6709\u5355\u4f4d\u90fd\u6b63\u786e\uff0c\u5b9e\u9a8c\u6761\u4ef6(\u5982\u65f6\u95f4\u3001\u6e29\u5ea6)\u90fd\u6e05\u695a\u3002\u6700\u540e\uff0c\u5982\u679c\u5f00\u5c55\u4e86\u7edf\u8ba1\u5b66\u5206\u6790\u6765\u8bc4\u4ef7\u7814\u7a76\u7ed3\u679c\u7684\u610f\u4e49\uff0c\u5219\u5e94\u5728\u201c\u65b9\u6cd5\u201d\u7684\u6700\u540e\u4e00\u6bb5\u53d9\u8ff0\u4f60\u7684\u7edf\u8ba1\u5b66\u65b9\u6cd5\uff0c\u5305\u62ec\u6240\u9009\u62e9\u7684\u663e\u8457\u6027\u9608\u503c\u3002\u5b9e\u4f8b\u201c\u201ehomogenates were spun at 10,000 \u00d7g and 4 \u00b0C for 12 min\u201d\u8fd9\u53e5\u8bdd\u5305\u542b\u4e86\u65f6\u95f4\u3001\u6e29\u5ea6\u4e24\u4e2a\u91cd\u8981\u7ec6\u8282\u3002\u800c\uff1a\u201c\u201ehomogenates were spun at 10,000 \u00d7g\u201d \u548c\u66f4\u7b80\u5316\u7684\u201chomogenates were centrifuged\u201d \u4e24\u4e2a\u53e5\u5b50\u4e2d\u5c31\u9057\u6f0f\u8fd9\u4e9b\u7ec6\u8282\u3002\u8fd9\u4e9b\u7ec6\u8282\u6709\u53ef\u80fd\u5bf9\u5f97\u5230\u4f60\u7684\u7ed3\u679c\u5f88\u91cd\u8981\uff0c\u6240\u4ee5\u5e94\u8be5\u7ed9\u51fa\u3002<\/p>\n
\u540c\u7406\uff0c\u4e0d\u8981\u53ea\u5199\uff1a\u201cThen, 10 \u03bcl of a propidium iodide solution was added to the\u00a0cells\u201d\uff0c\u56e0\u4e3a\u8fd9\u5bf9\u8bfb\u8005\u6beb\u65e0\u610f\u4e49\uff0c\u9664\u975e\u4ed6\u4eec\u77e5\u9053\u8be5\u6eb6\u6db2\u7684\u6d53\u5ea6\u3002\u5e94\u8be5\u5199\u6210\uff1a\u201cPropidium iodide was added to the\u00a0wells to a final concentration of 0.5 \u03bcg\/ml\u201d\u3002<\/p>\n
\u4e13\u4e1a\u671f\u520a\u4fa7\u91cd\u5f15\u8a00\u3001\u7ed3\u8bba\u3001\u8ba8\u8bba\uff0c\u90a3\u4e48\u8fd9\u4e9b\u90e8\u5206\uff0c\u6211\u4eec\u5e94\u8be5\u600e\u4e48\u5199\u5462\uff1f<\/p>\n
\u5f15\u8a00\u90e8\u5206\u4e3b\u8981\u56de\u7b54\u4e3a\u4ec0\u4e48\u7814\u7a76\uff0c\u4ecb\u7ecd\u8bba\u6587\u80cc\u666f\uff0c\u76f8\u5173\u9886\u57df\u7814\u7a76\u5386\u53f2\u4e0e\u73b0\u72b6\uff0c\u672c\u6587\u76ee\u7684\u610f\u4e49\uff0c\u521b\u65b0\u5728\u4ec0\u4e48\u5730\u65b9(\u6709\u5f85\u89e3\u51b3\u7684\u95ee\u9898)\u3002\u5f15\u8a00\u7b2c\u4e00\u53e5\u8bdd\u5f88\u91cd\u8981\uff0c\u5e94\u5f53\u660e\u786e\u63d0\u51fa\u8fd9\u7bc7\u6587\u7ae0\u7684\u76ee\u7684\uff0c\u5e76\u4e14\u8868\u793a\u76ee\u7684\u5f88\u91cd\u8981\u3002<\/p>\n
\u5f15\u8a00\u5305\u542b\u7684\u8981\u7d20\uff1a1\u3001\u6587\u7ae0\u7684\u76ee\u7684;2\u3001\u5bf9\u76ee\u7684\u7684\u8bc1\u5b9e(\u4e3a\u4ec0\u4e48\u6574\u4e2a\u5de5\u4f5c\u91cd\u8981);3\u3001\u80cc\u666f\uff0c\u5176\u4ed6\u4eba\u5df2\u7ecf\u505a\u4e86\u7684\uff0c\u600e\u6837\u53bb\u505a\u7684\uff0c\u6211\u4eec\u4ee5\u524d\u5df2\u7ecf\u505a\u7684;4\u3001\u6307\u5bfc\u4f5c\u8005\uff1a\u4f5c\u8005\u5e94\u8be5\u5728\u6587\u7ae0\u4e2d\u770b\u5230\u4ec0\u4e48?\u6587\u7ae0\u4e2d\u8ba9\u4eba\u611f\u5174\u8da3\u7684\u5173\u952e\u70b9\u662f\u4ec0\u4e48?\u6211\u4eec\u4f7f\u7528\u4e86\u4ec0\u4e48\uff0c\u6211\u4eec\u4f7f\u7528\u4ec0\u4e48\u65b9\u6cd5\u6765\u505a\u7684?\u672c\u6587\u91c7\u7528\u7684\u57fa\u672c\u65b9\u6cd5\u548c\u5047\u8bbe\uff1b5\u3001\u6982\u62ec\u548c\u603b\u7ed3\uff1a\u4f5c\u8005\u6240\u671f\u671b\u7684\u7ed3\u8bba\u662f\u4ec0\u4e48?<\/p>\n
\u7ed3\u8bba\u90e8\u5206\u5e94\u5f53\u9c9c\u660e\u51c6\u786e\u3001\u5b8c\u6574\u800c\u6709\u6761\u7406\uff0c\u5728\u524d\u6587\u6240\u9610\u8ff0\u7684\u4e8b\u5b9e\u6750\u6599\u7684\u57fa\u7840\u4e0a\uff0c\u8fdb\u884c\u5fc5\u8981\u7684\u63a8\u7406\u5224\u65ad\uff0c\u662f\u6574\u4e2a\u7814\u7a76\u5de5\u4f5c\u7684\u7ed3\u679c\u548c\u8bba\u70b9\u7684\u6982\u62ec\u3002\u51e1\u672a\u5f04\u6e05\u7684\u4f5c\u7528\u673a\u7406\u4e0d\u8981\u4e71\u52a0\u63a8\u6d4b\uff0c\u4e5f\u4e0d\u8981\u8fc7\u591a\u51fa\u73b0\u201c\u5927\u6982\u201d\u3001\u201c\u6216\u8bb8\u201d\u4e4b\u7c7b\u542b\u7cca\u4e0d\u6e05\u7684\u7ed3\u8bba\u3002\u6709\u7684\u7814\u7a76\u5de5\u4f5c\u4e0d\u80fd\u660e\u786e\u5bfc\u51fa\u7ed3\u8bba\uff0c\u53ef\u4e0d\u5199\u201c\u7ed3\u8bba\u201d\uff0c\u800c\u8fdb\u884c\u5fc5\u8981\u7684\u201c\u8ba8\u8bba\u201d\u3002<\/p>\n
\u5728\u64b0\u5199\u8bba\u6587\u7ed3\u8bba\u7684\u65f6\u5019\uff0c\u6211\u4eec\u8981\u505a\u5230\u4ee5\u4e0b\u7684\u4e09\u70b9\uff1a<\/p>\n
1.\u5f52\u7eb3\u6027\u8bf4\u660e\u7814\u7a76\u7ed3\u679c\u6216\u53d1\u73b0;<\/p>\n
2.\u7ed3\u8bba\u6027\u8bf4\u660e\u7ed3\u679c\u7684\u53ef\u80fd\u539f\u56e0\u3001\u673a\u7406\u6216\u610f\u4e49;<\/p>\n
3.\u524d\u77bb\u6027\u8bf4\u660e\u672a\u89e3\u51b3\u7684\u95ee\u9898\u3002<\/p>\n
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