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class=\"wp-block-heading\">\u4e09\u3001\u5982\u4f55\u5728\u5199\u4f5c\u4e2d\u5408\u7406\u89c4\u907f\u201c\u81ea\u6211\u6284\u88ad\u201d\uff1f<\/h3>\n\n\n\n<p>\u4ee5\u4e0b\u51e0\u70b9\u5efa\u8bae\uff0c\u80fd\u5e2e\u52a9\u60a8\u5728\u4fdd\u6301\u5b66\u672f\u4e25\u8c28\u7684\u524d\u63d0\u4e0b\uff0c\u6709\u6548\u964d\u4f4e\u91cd\u590d\u7387\uff1a<\/p>\n\n\n\n<h4 class=\"wp-block-heading\">1. <strong>\u907f\u514d\u539f\u53e5\u590d\u5236\u7c98\u8d34<\/strong><\/h4>\n\n\n\n<p>\u5373\u4f7f\u662f\u5f15\u7528\u81ea\u5df1\u8fc7\u5f80\u8bba\u6587\u4e2d\u7684\u65b9\u6cd5\u6216\u7406\u8bba\u63cf\u8ff0\uff0c\u4e5f\u5e94\u91cd\u65b0\u7ec4\u7ec7\u8bed\u8a00\uff0c\u6362\u4e00\u79cd\u8868\u8ff0\u65b9\u5f0f\uff0c\u505a\u5230\u5185\u5bb9\u76f8\u8fd1\u4f46\u4e0d\u843d\u5165\u6587\u5b57\u91cd\u590d\u3002<\/p>\n\n\n\n<p>\u4f8b\u5982\uff1a<\/p>\n\n\n\n<blockquote class=\"wp-block-quote is-layout-flow wp-block-quote-is-layout-flow\">\n<p>\u539f\u6587\uff1aWe used a three-step purification protocol including affinity, ion-exchange, and size exclusion chromatography.<\/p>\n<\/blockquote>\n\n\n\n<blockquote class=\"wp-block-quote is-layout-flow wp-block-quote-is-layout-flow\">\n<p>\u6539\u5199\uff1aThe protein was isolated using a combination of affinity, ion-exchange, and gel filtration methods, following the workflow established in our previous study [Ref].<\/p>\n<\/blockquote>\n\n\n\n<hr class=\"wp-block-separator has-alpha-channel-opacity\"\/>\n\n\n\n<h4 class=\"wp-block-heading\">2. \u6e05\u695a\u6807\u6ce8\u81ea\u5f15\u6587\u732e<\/h4>\n\n\n\n<p>\u5728\u5f15\u7528\u81ea\u5df1\u5df2\u53d1\u8868\u7684\u5de5\u4f5c\u65f6\uff0c\u52a1\u5fc5\u5728\u6587\u4e2d\u7528\u6e05\u6670\u7684\u65b9\u5f0f\u6807\u660e\u6765\u6e90\uff0c\u6bd4\u5982\uff1a<\/p>\n\n\n\n<blockquote class=\"wp-block-quote is-layout-flow wp-block-quote-is-layout-flow\">\n<p>\u201cAs demonstrated in our earlier study (Wang et al., 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