{"id":1830,"date":"2018-07-16T15:37:23","date_gmt":"2018-07-16T07:37:23","guid":{"rendered":"http:\/\/www.ithenticate.com.cn\/news\/?p=1830"},"modified":"2018-07-16T15:37:23","modified_gmt":"2018-07-16T07:37:23","slug":"%e6%8a%95%e7%a8%bf%e5%90%8e%ef%bc%9a%e5%af%b9%e5%be%85%e5%ae%a1%e7%a8%bf%e4%bf%ae%e7%a8%bf%e6%84%8f%e8%a7%81%e5%ba%94%e6%8c%81%e4%bb%80%e4%b9%88%e6%a0%b7%e7%9a%84%e6%80%81%e5%ba%a6%e5%91%a2%ef%bc%9f","status":"publish","type":"post","link":"https:\/\/www.ithenticate.com.cn\/news\/?p=1830","title":{"rendered":"\u6295\u7a3f\u540e\uff1a\u5bf9\u5f85\u5ba1\u7a3f\u4fee\u7a3f\u610f\u89c1\u5e94\u6301\u4ec0\u4e48\u6837\u7684\u6001\u5ea6\u5462\uff1f"},"content":{"rendered":"
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\u6280\u5de71\uff1a\u5148\u793c\u540e\u5175\uff0c\u793c\u8c8c\u5f00\u5934<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/article>\n<\/section>\n

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Thank you for your letter and for the reviewers\u2019 comments concerning our manuscript entitled \u201c\u6587\u7ae0\u9898\u76ee\u201d. These comments are all valuable and very helpful for revising and improving our paper, as well as the important guiding significance to our researches. We have studied comments carefully and have made corrections which we hope meet with approval. \uff08\u4f60\u7684\u63d0\u8bae\u592a\u6709\u7528\u4e86\uff0c\u6211\u8bba\u6587\u7684\u8d28\u91cf\u8e6d\u8e6d\u8e6d\u5c31\u4e0a\u53bb\u4e86\uff01\u6211\u4eec\u76f8\u5f53\u4ed4\u7ec6\u7814\u7a76\u4e86\u4f60\u7684\u5efa\u8bae\u4e4b\u7c7b\u7684\u8bdd\uff09The responds to the reviewer\u2019s comments are as follows: …….<\/p>\n<\/section>\n<\/section>\n
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\u56de\u590d\uff1aAs for the referee\u2019s concern, we have added scale bars to the Figures.<\/p>\n<\/section>\n<\/section>\n
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\u56de\u590d\uff1aAs for the referee\u2019s concern, we have indicated a significant difference with asterisk in figures.<\/p>\n<\/section>\n<\/section>\n
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\u56de\u590d\uff1aWe are very sorry for the mistakes in this manuscript and inconvenience they caused in your reading. The manuscript has been thoroughly revised and edited by a native speaker, so we hope it can meet the journal\u2019s standard. Thanks so much for your useful comments.<\/p>\n<\/section>\n<\/section>\n
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\u56de\u590d\uff1aAs for the referee\u2019s concern, the full descriptions of the abbreviations like IHC, MTT etc have been supplemented in the revised manuscript.<\/p>\n<\/section>\n<\/section>\n
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\u56de\u590d\uff1aWe are very sorry for the mistake. Lentivirus-transduced cells were washed twice with PBS, and suspended in a lysis buffer (2% mercaptoethanol, 20% glycerol, and 4% SDS, in 100 mM Tris-HCl buffer, pH 6.8). The information for the lysis buffer has been corrected in the materials and methods.<\/p>\n<\/section>\n<\/section>\n
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\u56de\u590d\uff1aIndeed, it will be more convincing if we get a comparative assessment on normal keratinocytes. However, the normal skin cell line TE 353.SK that we recently obtained could not be cultured stably under the condition of our lab at this time. We are also collecting human tissue samples from patients with primary melanoma and benign nevi. Based on our data, XXX expression will be analyzed in clinical samples by immunohistochemistry, and the correlation between XXX expression and the prognosis of melanoma will be determined in our subsequent investigations. Therefore, the referee\u2019s concern is of importance for our further study, and we will show the results in our next paper for XXX.<\/p>\n<\/section>\n<\/section>\n
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Tips\uff1a\u8865\u5145\u52a8\u7269\u5b9e\u9a8c\uff0c\u662f\u5ba1\u7a3f\u4eba\u6700\u7231\u53d1\u7684\u4e00\u62db\uff0c\u5176\u5b9e\u4e00\u70b9\u4e0d\u4ee4\u4eba\u610f\u5916\u3002\u65e2\u7136\u8eb2\u4e0d\u8fc7\uff0c\u5c31\u8981\u89c1\u62db\u62c6\u62db\uff0c\u5982\u679c\u80fd\u8865\u4e0a\uff0c\u5f53\u7136\u662f\u7ed9\u4e86\u6f02\u4eae\u7684\u56de\u9a6c\u67aa\uff1b\u8981\u662f\u6ca1\u6709\u6761\u4ef6\u8865\uff0c\u6216\u8005\u5b9e\u9a8c\u6765\u4e0d\u53ca\uff0c\u4e5f\u8981\u5c3d\u91cf\u8bf4\u7684\u59d4\u5a49\u70b9\uff0c\u5047\u88c5\u4f60\u786e\u5b9e\u52aa\u529b\u4e86\uff0c\u662f\u5ba1\u7a3f\u4eba\u7ed9\u7684\u65f6\u95f4\u592a\u77ed\u4e86\uff0c\u6211\u6839\u672c\u6765\u4e0d\u53ca\u554a\uff01<\/p>\n

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\u56de\u590d\uff1aWe are appreciative of the reviewer\u2019s suggestion. Indeed, it will be more profound if we get the relevant results in vivo. We have purchased 10 thymus- BALB\/c nude mice (female, age 4-6 weeks) from the Animal Center of Chinese Academy of Medical Sciences. Osteosarcoma xenografts were established in nude mice according to a previous report (Gao et al., 2009) \uff08\u6211\u4eec\u53bb\u4e70\u4e86\u82e5\u5e72\u53ea\u5c0f\u9f20\uff0c\u6309\u7167\u6587\u732e\u7684\u65b9\u6cd5\u64cd\u4f5c\uff09. Briefly, control lentivirus or XXX gene shRNA-expressing lentivirus infected XXX cells (1\u00d7105) were suspended in 0.1 ml PBS and then injected subcutaneously into the proximal tibia of each anesthetized nude mice (n=5 in each group). Unfortunately, the xenografts could not grow after inoculation for three weeks \uff08\u6ee1\u6000\u6b23\u559c\u7684\u63a5\u79cd\u5230\u52a8\u7269\u8eab\u4e0a\uff0c\u7136\u800c\u5b9e\u9a8c\u8fd9\u4e1c\u897f\uff0c\u54ea\u80fd\u4e00\u904d\u5c31\u6210\u5462\uff0c\u6211\u4eec\u505a\u4e86\u4e00\u904d\uff0c\u7ed3\u679c\u6ca1\u80fd\u6210\u7624\uff0c\u5b9e\u5728\u662f\u60b2\u5267\u4e86\uff09. The limitation of technical condition may be the main reason. Also, we doubt that the cell viability could be insufficient to initiate osteosarcoma tumorigenesis in vivo due to the long-distance transport. Therefore, we seek for the editor\u2019s tolerance and understanding. Many thanks for your kind help!<\/p>\n<\/section>\n<\/section>\n

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